Composite
Part:BBa_K2957031:Design
Designed by: Melody Wu Group: iGEM19_MIT (2019-10-19)
TRE, CCL5
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2943
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2943
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2943
Illegal NgoMIV site found at 1479
Illegal AgeI site found at 2949 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2623
Illegal BsaI.rc site found at 2921
Illegal SapI site found at 402
Illegal SapI.rc site found at 2560
Design Notes
This part was created with Type IIS Cloning and transfected into HEKs. We tested this with Jurkat cells. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.
Source
Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.