Composite

Part:BBa_K2957031:Design

Designed by: Melody Wu   Group: iGEM19_MIT   (2019-10-19)


TRE, CCL5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2943
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2943
    Illegal NgoMIV site found at 1479
    Illegal AgeI site found at 2949
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2623
    Illegal BsaI.rc site found at 2921
    Illegal SapI site found at 402
    Illegal SapI.rc site found at 2560


Design Notes

This part was created with Type IIS Cloning and transfected into HEKs. We tested this with Jurkat cells. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>.


Source

Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.

References